Significant engineering of Saccharomyces cerevisiae is required to enable consolidated bioprocessing (CBP) of lignocellulose to ethanol. Genome modification in S. cerevisiae has been successful partly due to its efficient homology-directed DNA repair machinery. and CRISPR technology has made multi-gene editing even more accessible. https://www.roneverhart.com/Atma-s-Wail-East-Non-Ladder/